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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: High IGKC-Expressing Intratumoral Plasma Cells Predict Response to Immune Checkpoint Blockade
doi: 10.3390/ijms23169124
Figure Lengend Snippet: Multispectral Immunofluorescence tissue imaging of good and bad responder cases. ( a , b ) Fluorescence panels images of the markers CD11b, CD68, CD3, CD8, CD20, MELAN-A in ( a ) and CD19, CD20 and C138 in ( b ) of the patient IMK-20 (bad responder) and IMK-38 (good responder). ( c ) Boxplot with the Wilcoxon test comparing the density (Cells/mm 2 ) between good and bad responders of CD8 and CD19.
Article Snippet: The myeloid and lymphoid cell panel included: CD11b (Rabbit monoclonal, clone EPR1344, 1:1000, Abcam (Cambridge, UK), product number ab133357), CD68 (Mouse monoclonal, clone PG-M1, ready-to-use, Agilent, Santa Clara, CA 95051, USA, product number IR613), CD3 (Rabbit polyclonal, IgG, ready-to-use, Agilent, Santa Clara, CA 95051, USA, product number IR503), CD8 (Mouse monoclonal, clone C8/144B, ready-to-use, Agilent, Santa Clara, CA 95051, USA, product number GA62361-2), CD20 (Mouse monoclonal, IgG2α, clone L26, ready-to-use, Agilent, Santa Clara, CA 95051, USA, product number GA604) and
Techniques: Immunofluorescence, Imaging, Fluorescence
Journal: Romanian Journal of Morphology and Embryology
Article Title: Fortuitous discovery of melanomas in the ENT Department – a histopathological and immunohistochemical study
doi: 10.47162/RJME.61.4.18
Figure Lengend Snippet: Intensely positive Melan A cytoplasmic immunoreaction. Anti-Melan A antibody immunomarking, ×400
Article Snippet: The histopathological (HP) diagnosis was completed using IHC reactions for further antibodies: anti-Ki67 (monoclonal mouse anti-human Ki67, clone MM1, 1/200 dilution, Novocastra™, Leica Biosystems), anti-S100 protein (polyclonal rabbit anti-human S100 protein, clone EP32, 1/100 dilution, Novocastra™, Leica Biosystems), anti-HMB45 (monoclonal mouse anti-human HMB45, clone HMB45, 1/60 dilution, Novocastra™, Leica Biosystems),
Techniques:
Journal: Romanian Journal of Morphology and Embryology
Article Title: Fortuitous discovery of melanomas in the ENT Department – a histopathological and immunohistochemical study
doi: 10.47162/RJME.61.4.18
Figure Lengend Snippet: Intense Melan A positivity in tumor cells of cytoplasmic immunoreaction. Anti-Melan A antibody immunomarking, ×400
Article Snippet: The histopathological (HP) diagnosis was completed using IHC reactions for further antibodies: anti-Ki67 (monoclonal mouse anti-human Ki67, clone MM1, 1/200 dilution, Novocastra™, Leica Biosystems), anti-S100 protein (polyclonal rabbit anti-human S100 protein, clone EP32, 1/100 dilution, Novocastra™, Leica Biosystems), anti-HMB45 (monoclonal mouse anti-human HMB45, clone HMB45, 1/60 dilution, Novocastra™, Leica Biosystems),
Techniques:
Journal: Cells
Article Title: Extracellular Vesicles from Ocular Melanoma Have Pro-Fibrotic and Pro-Angiogenic Properties on the Tumor Microenvironment
doi: 10.3390/cells11233828
Figure Lengend Snippet: Exosomal and melanocytic markers present in UM-derived EVs. Cell pellets and EV fractions isolated from UM cell lines TJU-UM001 (metastatic), Mµ2 (non-metastatic), and Mµ2F (metastatic), or NCMs, were analyzed for their expression of exosomal (CD9, CD63, CD81, Tsg101) or melanocytic (Melan-A, TYRP1) markers by Western blotting. The anti-α-tubulin was used as marker of cytosolic proteins. Ponceau S staining was used as a loading control. Arrows indicate the specific band for each marker.
Article Snippet: Membranes were blocked for 1 h at room temperature with phosphate-buffered saline (PBS) 1X-5% non-fat milk-0.1% Tween ® 20 was then incubated overnight at 4 °C with the following primary antibodies: mouse anti-α-tubulin antibody (clone DM1A, dilution 1:2500; Abcam), mouse anti-human CD9 tetraspanin antibody (clone HI9a, dilution 1:1000; BioLegend), mouse anti-human CD63 tetraspanin antibody (clone H5C6, dilution 1:1000; BioLegend), mouse anti-human CD81 tetraspanin antibody (clone 5A6, dilution 1:1000; Abcam),
Techniques: Derivative Assay, Isolation, Expressing, Western Blot, Marker, Staining